Answer: In case of assays with RNA as the nucleic acid, reverse transcription PCR (RT PCR) is performed. The Truenat pouch contains Microtube with freeze dried RT PCR reagents. Six (6) μL of the purified RNA is then dispensed using the provided micropipette and tip into the microtube containing freeze dried RT PCR reagents, including reverse transcriptase (RT) and allowed to stand for 30-60 seconds to get a clear solution. Six (6) μL of this clear solution is then pipetted out using the same pipette and tip and dispensed into the reaction well of the Truenat disease specific chip and the test is inserted in the Truelab Real Time micro PCR Analyzer where the RNA is first converted into complementary DNA (cDNA) by the RT enzyme and further thermal cycling takes place.
Answer : Ct (Threshold cycle) reflects the the cycle number at which the fluorescence signal generated within the reaction crosses the threshold. It is inversely correlated to the logarithm of the initial copy number. The Ct assigned to a particular sample thus reflects the point during the reaction at which a sufficient number of amplicons have accumulated. It is also sometimes called the crossing over (Cp) point.
Answer : No, every lot of the Truenat chip comes with the in-built Standard curve values. So when you run the Truenat chip you do not require to run a standard curve every time.
Answer : Yes, the Stored data on the Truelab Real Time micro PCR analyzer can be exported via Bluetooth, Email or can be transferred to a computer by connecting a USB cable to the Truelab Device. The device also comes with a micro SIM card. The results obtained also then can be sent via SMS.
Answer :The Truelab Real Time Workstation comes along with the Truelab Micro PCR printer. The Printer is connected to Truelab Device via a Bluetooth connection. The results obtained on the Truelab Device can be then printed by giving a print command on the result page of the Truelab Real time micro PCR analyzer.